Pde4b^tm1Mct
Targeted Allele Detail

Summary

• Symbol: Pde4b^tm1Mct
• Name: phosphodiesterase 4B, cAMP specific; targeted mutation 1, Marco Conti
• MGI ID: MGI:2181444
• Gene: Pde4b Location: Chr4:101944740-102464456 bp, + strand Genetic Position: Chr4, 46.99 cM
• Alliance: Pde4b^tm1Mct page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76862
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of a 3 kb region encoding exons 8-10 with a PGK-hprt cassette (in the reverse orientation) via homologous recombination. The gene targeting event does not disrupt transcription initiation and mutant transcripts were detected by RT-PCR in homozygous mutant animals. Western blot analysis using antibodies directed against the C-terminus of the protein failed to detect protein product in the cerebellum of homozygous mutants. (J:76862)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Pde4b Mutation: 55 strains or lines available

References

• Original: J:76862 Jin SL, et al., Induction of the cyclic nucleotide phosphodiesterase PDE4B is essential for LPS-activated TNF-alpha responses. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7628-33
• All: 21 reference(s)