Cnn1^tm1Tkhi
Targeted Allele Detail

Summary

• Symbol: Cnn1^tm1Tkhi
• Name: calponin 1; targeted mutation 1, Katsuhito Takahashi
• MGI ID: MGI:2181445
• Gene: Cnn1 Location: Chr9:22010501-22020517 bp, + strand Genetic Position: Chr9, 8.3 cM, cytoband A2-A4
• Alliance: Cnn1^tm1Tkhi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77112
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A gpt-neo cassette replaced exons 5 through 7, encoding the regulatory binding region, conserved tandem repeats, and a carboxyl-terminal domain specific to the basic Calponin. RT-PCR analysis of homozygous mutant embryos showed an absence of basic transcript in both healthy and healing bone tissue. Western blot analysis confirmed an absence of the basic protein in homozygous mutant mice. (J:77112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnn1 Mutation: 17 strains or lines available

References

• Original: J:77112 Yoshikawa H, et al., Mice lacking smooth muscle calponin display increased bone formation that is associated with enhancement of bone morphogenetic protein responses. Genes Cells. 1998 Oct;3(10):685-95
• All: 9 reference(s)