Cenpb^tm1Khac
Targeted Allele Detail

Summary

• Symbol: Cenpb^tm1Khac
• Name: centromere protein B; targeted mutation 1, K H Andy Choo
• MGI ID: MGI:2181521
• Synonyms: +/- neo, R1^-
• Gene: Cenpb Location: Chr2:131019209-131021974 bp, - strand Genetic Position: Chr2, 63.29 cM
• Alliance: Cenpb^tm1Khac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47171
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Stop codons were introduced into all three reading frames of the intronless gene by the insertion a translation frameshift linker into the amino-terminal centromere DNA-binding domain. An IRES-neomycin selection marker was inserted in the 3' untranslated region upstream of the poly adenylation signal. RT-PCR analysis of heterozygous mutant mice showed the expression of a truncated transcript. Mice possessing both this allele and Cenpb^tm2Khac were shown to lack transcript and protein was not detected by immunofluorescence staining of metaphase chromosomes. (J:47171)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpb Mutation: 18 strains or lines available

References

• Original: J:47171 Hudson DF, et al., Centromere protein B null mice are mitotically and meiotically normal but have lower body and testis weights. J Cell Biol. 1998 Apr 20;141(2):309-19
• All: 3 reference(s)