Phlda2^tm2Bty
Targeted Allele Detail

Summary

• Symbol: Phlda2^tm2Bty
• Name: pleckstrin homology like domain, family A, member 2; targeted mutation 2, Benjamin Tycko
• MGI ID: MGI:2181543
• Synonyms: Ipl^loxP
• Gene: Phlda2 Location: Chr7:143055282-143056882 bp, - strand Genetic Position: Chr7, 88.23 cM
• Alliance: Phlda2^tm2Bty page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76871
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination introduced loxP sites flanking the 2 exons of the gene. Mice carrying the targeted mutation were bred to the HSP-cre deleter strain resulting in deletion of the 2 exons and of half of the 5' upstream CpG islands. Absence of gene expression in yolk sacs of homozygous mutant embryos was demonstrated by Northern blot analysis. (J:76871)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phlda2 Mutation: 12 strains or lines available

References

• Original: J:76871 Frank D, et al., Placental overgrowth in mice lacking the imprinted gene Ipl. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7490-5
• All: 4 reference(s)