Nodal^tm2Rob
Targeted Allele Detail

Summary

• Symbol: Nodal^tm2Rob
• Name: nodal growth differentiation factor; targeted mutation 2, Elizabeth J Robertson
• MGI ID: MGI:2181583
• Synonyms: Nodal^D600, nodal^delta600
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm2Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56145
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: By homologous recombination in ES cells heterozygous for the Nodal^tm1Rob allele, the entire 600 bp ASE enhancer region was replaced by a loxP-flanked hygromycin selection cassette in trans to the original mutation. Transient expression of Cre recombinase in targeted ES cells resulted in excision of the selection cassette, leaving a single loxP site in place of the enhancer sequences. Subsequent matings of mice carrying this allele resulted in segregation from the Nodal^tm1Rob allele. (J:77125)

Expression

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 42 strains or lines available

References

• Original: J:56145 Norris DP, et al., Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements. Genes Dev. 1999 Jun 15;13(12):1575-88
• All: 7 reference(s)