Prkg1^tm1Hfm
Targeted Allele Detail

Summary

• Symbol: Prkg1^tm1Hfm
• Name: protein kinase, cGMP-dependent, type I; targeted mutation 1, Franz Hofmann
• MGI ID: MGI:2181586
• Synonyms: cGKI-, cGKI-KO, cGKI^L, PKG-I^-
• Gene: Prkg1 Location: Chr19:30541889-31742433 bp, - strand Genetic Position: Chr19, 25.17 cM, cytoband C2
• Alliance: Prkg1^tm1Hfm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48058
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The exon encoding a portion of the ATP-binding domain, which is common to both known isoforms, was replaced by a neomycin selection cassette. Northern blot analysis showed an absence of transcript in homozygous mutant uterine tissue. Western blot analysis of platelets, aorta, and duodenum from homozygous mutant mice showed that while Prkg1 protein was absent, levels of Prkg2 protein were unaffected. cGMP-stimulated kinase activity was undtected in lung extracts from homozygous mutant mice. (J:48058)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkg1 Mutation: 60 strains or lines available

References

• Original: J:48058 Pfeifer A, et al., Defective smooth muscle regulation in cGMP kinase I-deficient mice. EMBO J. 1998 Jun 1;17(11):3045-51
• All: 21 reference(s)