P2rx1^tm1Chn
Targeted Allele Detail

Summary

• Symbol: P2rx1^tm1Chn
• Name: purinergic receptor P2X, ligand-gated ion channel, 1; targeted mutation 1, R Channel
• MGI ID: MGI:2181608
• Synonyms: P2X1-, P2X1-receptor-
• Gene: P2rx1 Location: Chr11:72889929-72906026 bp, + strand Genetic Position: Chr11, 45.09 cM
• Alliance: P2rx1^tm1Chn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59336
• Parent Cell Line: E14.1a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, encoding the first 45 amino acids, and surrounding intronic sequence were replaced using a vector containing lacZ and neo. Transcript was undetected by RT-PCR analysis of homozygous mutant mice. Western blot analysis, using an antibody raised against the carboxy-terminal, showed an absence of protein in homozygous mutant mice. A lack of immunoreactivity was observed in sections of the vas deferens obtained from homozygous mutant mice. (J:59336)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2rx1 Mutation: 29 strains or lines available

References

• Original: J:59336 Mulryan K, et al., Reduced vas deferens contraction and male infertility in mice lacking P2X1 receptors [see comments]. Nature. 2000 Jan 6;403(6765):86-9
• All: 12 reference(s)