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Lipetm1Ishi
Targeted Allele Detail
Summary
Symbol: Lipetm1Ishi
Name: lipase, hormone sensitive; targeted mutation 1, Shun Ishibashi
MGI ID: MGI:2181610
Synonyms: HSL-, HSL-KO
Gene: Lipe  Location: Chr7:25078952-25097911 bp, - strand  Genetic Position: Chr7, 13.78 cM, cytoband A3
Alliance: Lipetm1Ishi page
Lipetm1Ishi/Lipetm1Ishi males exhibit oligospermia and seminiferous tubule defects

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:59889
Parent Cell Line:  JH1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsPortions of exon 5 and exon 6, as well as the intervening intron, were replaced with a neo cassette. The deleted region encoded the catalytic domain. Northern blot analysis of epididymal fat obtained from homozygous mutant mice showed a lack of normal transcript. An absence of protein in homozygous mutant mice was demonstrated by Western blot analysis of the epididymal fat and testes. Neutral cholesterol ester hydrolase (NCEH) activity was undetected by assays involving white and brown adipose tissue. (J:59889)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lipe Mutation:  37 strains or lines available
References
Original:  J:59889 Osuga J, et al., Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity [see comments]. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):787-92
All:  20 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory