Lif^tm1Phb
Targeted Allele Detail

Summary

• Symbol: Lif^tm1Phb
• Name: leukemia inhibitory factor; targeted mutation 1, Philippe Brulet
• MGI ID: MGI:2181618
• Synonyms: LIF(-), LIF^-, LIF KO
• Gene: Lif Location: Chr11:4207587-4222514 bp, + strand Genetic Position: Chr11, 2.94 cM, cytoband A1-A2
• Alliance: Lif^tm1Phb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77410
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3.3 kb region, encompassing all of the exonic and intronic sequence as well as a portion of the 3' untranslated region, was replaced by a vector containing lacZ. Sequence analysis confirmed the junction of the endogenous promoter with the lacZ start codon. (J:77410)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lif Mutation: 11 strains or lines available

References

• Original: J:77410 Escary JL, et al., Leukaemia inhibitory factor is necessary for maintenance of haematopoietic stem cells and thymocyte stimulation. Nature. 1993 May 27;363(6427):361-4
• All: 29 reference(s)