Hprt1^{tm1(CAG-cre)Mnn}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CAG-cre)Mnn}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Jeffrey R Mann
• MGI ID: MGI:2181632
• Synonyms: Hprt1^tm1(cre)Mnn, hprt-cre, Hprt^cre, Hprt^{tm1(CAG.cre)MdCoh}
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CAG-cre)Mnn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75826
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hprt1^{tm1(CAG-cre)Mnn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: An expression cassette in which a CMV enhancer and chicken beta-globin promoter driving Cre recombinase expression was inserted in reverse transcriptional orientation into exon 3 of the gene. A PGK-neomycin selection cassette was also inserted following the cre gene. Expression of Cre recombinase occurred at high levels in the female germline and fertilized eggs, and all offspring of heterozyous females display excision of reporter sequences. (J:75826)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Disease models

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:75826 Tang SH, et al., A cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ. Genesis. 2002 Mar;32(3):199-202
• All: 161 reference(s)