Slc12a2^tm1Bhk
Targeted Allele Detail

Summary

• Symbol: Slc12a2^tm1Bhk
• Name: solute carrier family 12, member 2; targeted mutation 1, Beverly H Koller
• MGI ID: MGI:2181636
• Synonyms: Slc12a2^{delta1065-1137}
• Gene: Slc12a2 Location: Chr18:58011750-58079893 bp, + strand Genetic Position: Chr18, 32.15 cM
• Alliance: Slc12a2^tm1Bhk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60671
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 24 and 25, encoding amino acids in the carboxyl-terminal cytoplasmic domain (residues 1065 through 1137), were replaced with a neomycin selection cassette. Northern blot analysis of salivary gland total RNA showed normal levels of a truncated transcript. RT-PCR analysis, using probes flanking the targeted region indicated a deletion of 195 coding-nucleotides in mutant mice. (J:60671)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a2 Mutation: 58 strains or lines available

References

• Original: J:60671 Pace AJ, et al., Failure of spermatogenesis in mouse lines deficient in the Na(+)-K(+)-2Cl(-) cotransporter. J Clin Invest. 2000 Feb;105(4):441-50
• All: 4 reference(s)