Bace1^tm1.1Slr
Targeted Allele Detail

Summary

• Symbol: Bace1^tm1.1Slr
• Name: beta-site APP cleaving enzyme 1; targeted mutation 1.1, Steven L Roberds
• MGI ID: MGI:2181644
• Synonyms: BACE^-, exon 4-8-deleted
• Gene: Bace1 Location: Chr9:45749878-45775694 bp, + strand Genetic Position: Chr9, 25.22 cM
• Alliance: Bace1^tm1.1Slr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77414
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated by breeding mice heterozygous for Bace^tm1Slr and transgenic mice constituitively expressing FLP recombinase. Exons 4 through 8 as well as the neomycin selection cassette and the FLP recombinase target (FRT) sites flanking it were excised. The deleted exons encoded two active site aspartate residues. One FRT site remained in intron 8. Beta-secretase activity was undetected in extracts of cortical cell cultures derived from homozygous mutant animals. (J:77414)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bace1 Mutation: 30 strains or lines available

References

• Original: J:77414 Roberds SL, et al., BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics. Hum Mol Genet. 2001 Jun 1;10(12):1317-24
• All: 4 reference(s)