Bace1^tm2Slr
Targeted Allele Detail

Summary

• Symbol: Bace1^tm2Slr
• Name: beta-site APP cleaving enzyme 1; targeted mutation 2, Steven L Roberds
• MGI ID: MGI:2181645
• Synonyms: exon 1-disrupted
• Gene: Bace1 Location: Chr9:45749878-45775694 bp, + strand Genetic Position: Chr9, 25.22 cM
• Alliance: Bace1^tm2Slr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77414
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An expression cassette, containing a beta-galactosidase gene adjoined to an IRES as well as a neomycin resistance gene adjoined to a MC1 promoter, was inserted 2 nucleotides downstream of the start codon. The deleted sequence extended through the end of exon 1, a total of 165 nucleotides. Beta-secretase activity was undetected in brain homogenates and extracts of cortical cell cultures derived from homozygous mutant animals. (J:77414)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bace1 Mutation: 30 strains or lines available

References

• Original: J:77414 Roberds SL, et al., BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics. Hum Mol Genet. 2001 Jun 1;10(12):1317-24
• All: 3 reference(s)