Usf2^tm1Msw
Targeted Allele Detail

Summary

• Symbol: Usf2^tm1Msw
• Name: upstream transcription factor 2; targeted mutation 1, Michele Sawadogo
• MGI ID: MGI:2181680
• Synonyms: Usf2(-)
• Gene: Usf2 Location: Chr7:30645421-30656288 bp, - strand Genetic Position: Chr7, 19.27 cM, cytoband A2-B1
• Alliance: Usf2^tm1Msw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46788
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 11 kb of the gene, including most of the 5' untranslated region and all of the coding exons, was deleted and replaced with a PGK-neo cassette via homologous recombination. The absence of gene expression in homozygous mutants was confirmed by electrophoretic mobility shift assay (EMSA) and Western blot analysis of nuclear extracts from mutant mouse embryonic fibroblasts (MEF). (J:46788)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usf2 Mutation: 12 strains or lines available

References

• Original: J:46788 Sirito M, et al., Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice. Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3758-63
• All: 6 reference(s)