Egr1^tm1Pch
Targeted Allele Detail

Summary

• Symbol: Egr1^tm1Pch
• Name: early growth response 1; targeted mutation 1, Patrick Charnay
• MGI ID: MGI:2181755
• Synonyms: Egr-1^-LacZ), Krox-24-, Zif268-
• Gene: Egr1 Location: Chr18:34994260-34998009 bp, + strand Genetic Position: Chr18, 18.76 cM, cytoband C/D
• Alliance: Egr1^tm1Pch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45064
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A construct containing a promoterless lacZ gene adjoined to a neomycin selection gene was inserted 50 bp upstream of the translational start codon. The insertion introduced a frameshift mutation upstream of the DNA-binding domain. An absence of encoded mRNA and protein was determined by in situ hybridization and immunocytochemical analyses of homozygous mutant mice. (J:45064)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Egr1 Mutation: 21 strains or lines available

References

• Original: J:45064 Topilko P, et al., Multiple pituitary and ovarian defects in Krox-24 (NGFI-A, Egr-1)-targeted mice. Mol Endocrinol. 1998 Jan;12(1):107-22
• All: 21 reference(s)