Cbx2^tm1Ykf
Targeted Allele Detail

Summary

• Symbol: Cbx2^tm1Ykf
• Name: chromobox 2; targeted mutation 1, Yuko Katoh-Fukui
• MGI ID: MGI:2181776
• Synonyms: Cbx2^-, M33^cterm
• Gene: Cbx2 Location: Chr11:118913845-118922101 bp, + strand Genetic Position: Chr11, 83.33 cM
• Alliance: Cbx2^tm1Ykf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48123
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The targeted locus was disrupted by the insertion of a neomycin selection cassette into exon 5. Transcripts 1.5 kb longer than wild-type, putatively resulting from the incorporation of the neo transgene, were detected in homozygous mutant embryos via Northern blot analysis. Western blot analysis of lysates obtained from homozygous mutant embryos showed a lack of normal protein. The absence of a truncated peptide encoded by sequence upstream of the insertion could not be confirmed due to the lack of an appropriate antibody. (J:48123)

Expression

In Mice Carrying this Mutation:	14 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Cbx2 Mutation: 38 strains or lines available

References

• Original: J:48123 Katoh-Fukui Y, et al., Male-to-female sex reversal in M33 mutant mice. Nature. 1998 Jun 18;393(6686):688-92
• All: 7 reference(s)