Plec^tm2Gwi
Targeted Allele Detail

Summary

• Symbol: Plec^tm2Gwi
• Name: plectin; targeted mutation 2, Gerhard Wiche
• MGI ID: MGI:2181792
• Synonyms: exon 2-4-deficient
• Gene: Plec Location: Chr15:76055174-76115578 bp, - strand Genetic Position: Chr15, 35.48 cM
• Alliance: Plec^tm2Gwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59000
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 4 were replaced by a neomycin selection cassette. The 5' end of exon 2 serves as a splice-acceptor site utilized in all four identified alternative transcripts. Transcript was undetected by RT-PCR analysis of samples derived from homozygous mutant skin and muscle tissue. An absence of protein was determined by immunoblot analysis of skin and muscle tissue derived from homozygous mutant mice. (J:59000)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plec Mutation: 174 strains or lines available

References

• Original: J:59000 Andra K, et al., Targeted inactivation of plectin reveals essential function in maintaining the integrity of skin, muscle, and heart cytoarchitecture. Genes Dev. 1997 Dec 1;11(23):3143-56
• All: 4 reference(s)