Mest^tm1Masu
Targeted Allele Detail

Summary

• Symbol: Mest^tm1Masu
• Name: mesoderm specific transcript; targeted mutation 1, M Azim Surani
• MGI ID: MGI:2181803
• Synonyms: Mest^+/-, Mest^tm1Lef, Mest^tm1Lef-pat
• Gene: Mest Location: Chr6:30723546-30748464 bp, + strand Genetic Position: Chr6, 12.53 cM, cytoband B1
• Alliance: Mest^tm1Masu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50170
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encompassing all of exons 3 through 8 and a portion of exon 9 was replaced with a cassette containing an IRES-lacZ-neo fusion gene. RT-PCR analysis of total embryonic RNA showed an absence of normal transcript in animals possessing a targeted paternal allele and a silenced wild-type maternal allele. Paternal transmission of this allele yielded a lacZ expression pattern similar to that of the endogenous gene. (J:50170)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mest Mutation: 27 strains or lines available

References

• Original: J:50170 Lefebvre L, et al., Abnormal maternal behaviour and growth retardation associated with loss of the imprinted gene Mest [see comments]. Nat Genet. 1998 Oct;20(2):163-9
• All: 8 reference(s)