Rho^tm1Phm
Targeted Allele Detail

Summary

• Symbol: Rho^tm1Phm
• Name: rhodopsin; targeted mutation 1, Peter Humphries
• MGI ID: MGI:2181812
• Synonyms: R^-, Rho-
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^tm1Phm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38098
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin cassette under the control of a polymerase II promoter was inserted at codon 135 in exon 2. RT-PCR analysis of total RNA derived from ocular tissue showed an absence of transcript in homozygous mutant mice. A lack of encoded protein was determined in homozygous mutant mice via immunocytochemical staining of retinal sections. (J:38098)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 51 strains or lines available

References

• Original: J:38098 Humphries MM, et al., Retinopathy induced in mice by targeted disruption of the rhodopsin gene [see comments]. Nat Genet. 1997 Feb;15(2):216-9
• All: 78 reference(s)