Csn2^tm1Psim
Targeted Allele Detail

Summary

• Symbol: Csn2^tm1Psim
• Name: casein beta; targeted mutation 1, Paul Simons
• MGI ID: MGI:2182074
• Synonyms: csnb^-
• Gene: Csn2 Location: Chr5:87840478-87847288 bp, - strand Genetic Position: Chr5, 43.56 cM
• Alliance: Csn2^tm1Psim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:18947
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 2 immediately downstream of the translational start codon. The disrupted exon encoded the secretion signal. Northern blot analysis showed an absence of transcript in the mammary glands of homozygous mutant mice. The encoded protein was not detected by Wester blot analysis of milk obtained from homozygous mutant mice. (J:18947)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Csn2 Mutation: 18 strains or lines available

References

• Original: J:18947 Kumar S, et al., Milk composition and lactation of beta-casein-deficient mice [published erratum appears in Proc Natl Acad Sci U S A 1994 Nov 22;91(24):11767]. Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6138-42
• All: 1 reference(s)