Cst3tm1Karl
Targeted Allele Detail
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Symbol: |
Cst3tm1Karl |
Name: |
cystatin C; targeted mutation 1, S Karlsson |
MGI ID: |
MGI:2182140 |
Synonyms: |
Cstc-, CstC-, Cys68, CysC- |
Gene: |
Cst3 Location: Chr2:148713642-148717432 bp, - strand Genetic Position: Chr2, 73.6 cM
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Alliance: |
Cst3tm1Karl page
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Germline Transmission: |
Earliest citation of germline transmission:
J:62407
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Parent Cell Line: |
CCE/EK.CCE (ES Cell)
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Strain of Origin: |
129S/SvEv-Gpi1c
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: An attempted knockin resulted in the generation of a null mouse. The majority of exon 1 was replaced by a human Cst3 cDNA fragment containing a missense mutation that substitutes a glutamine for a leucine at codon 68. In addition, a neomycin selection cassette was inserted at the 3' end of exon 1 and replaced two thirds of intron 1. Northern blot analysis using probes for both the human and mouse transcript showed an absence of both transcripts in lung, heart, testis, kidney and brain tissues obtained from homozygous mutant mice. ELIZA using human- and mouse-specific antibodies failed to detect either protein in homozygous mutant animals.
(J:62407)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cst3 Mutation: |
14 strains or lines available
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Original: |
J:62407 Huh CG, et al., Decreased metastatic spread in mice homozygous for a null allele of the cystatin C protease inhibitor gene. Mol Pathol. 1999 Dec;52(6):332-40 |
All: |
18 reference(s) |
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