Ncan^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Ncan^tm1Ref
• Name: neurocan; targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:2182148
• Synonyms: NCIg
• Gene: Ncan Location: Chr8:70545735-70573494 bp, - strand Genetic Position: Chr8, 34.15 cM
• Alliance: Ncan^tm1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70858
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3, which encodes the immunoglobulin-like domain. Transcript was detected in homozygous mutant mice via Norhtern blot analysis. RT-PCR revealed that the detected signal was that of an aberrant transcript from which exon 3 had been excised. Western blot analysis showed an absence of protein. (J:70858)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncan Mutation: 46 strains or lines available

References

• Original: J:70858 Zhou XH, et al., Neurocan is dispensable for brain development. Mol Cell Biol. 2001 Sep;21(17):5970-8
• All: 2 reference(s)