Elane^tm1(cre)Roes
Targeted Allele Detail

Summary

• Symbol: Elane^tm1(cre)Roes
• Name: elastase, neutrophil expressed; targeted mutation 1, Jurgen Roes
• MGI ID: MGI:2182177
• Synonyms: Ela^Cre, GE^-, GEcre, NE^-
• Gene: Elane Location: Chr10:79722146-79724050 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Elane^tm1(cre)Roes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77597
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Elane^tm1(cre)Roes expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Elane (MGI:2679229)

• Mutation details: A construct containing a cre recombinase gene, in which GC pairs had been deleted, and a floxed neo gene, was incoporated at the start codon of the endogenous gene. The floxed neo gene was excised in vitro by transiently transfecting ES cells with cre expression vectors. Neither transcript nor protein were detected by Northern and Western blot analyses. Expression of the modified cre transgene permits conditional mutagenesis at sites tagged with loxP in myeloid precursor cells. (J:77597)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Elane (mouse) Summary of all recombinase alleles driven by Elane. Alliance mouse Elane gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Elane Mutation: 24 strains or lines available

References

• Original: J:77597 Tkalcevic J, et al., Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G. Immunity. 2000 Feb;12(2):201-10
• All: 22 reference(s)