Mesp2^{tm2(Mesp1)Ysa}
Targeted Allele Detail

Summary

• Symbol: Mesp2^{tm2(Mesp1)Ysa}
• Name: mesoderm posterior 2; targeted mutation 2, Yumiko Saga
• MGI ID: MGI:2182179
• Synonyms: Mesp2^P1
• Gene: Mesp2 Location: Chr7:79460475-79463179 bp, + strand Genetic Position: Chr7, 45.18 cM
• Alliance: Mesp2^{tm2(Mesp1)Ysa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49903
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A construct containing a downstream neomycin resistance gene and Mesp1 cDNA containing the entire coding region positioned at the start site of the Mesp2 gene was inserted into the Mesp2 locus. A non-coding region of Mesp2 was left intact and utilized for RT-PCR analysis, which showed that transcript from the Mesp2 locus was absent, while transcript from both the endogenous and transgenic Mesp1 loci were present. (J:49903)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mesp2 Mutation: 30 strains or lines available

References

• Original: J:49903 Saga Y, Genetic rescue of segmentation defect in MesP2-deficient mice by MesP1 gene replacement. Mech Dev. 1998 Jul;75(1-2):53-66
• All: 2 reference(s)