Tg(Col2a1-PTHLH)1Ecw
Transgene Detail

Summary

• Symbol: Tg(Col2a1-PTHLH)1Ecw
• Name: transgene insertion 1, Eleanor C Weir
• MGI ID: MGI:2182284
• Synonyms: col II-PTHrP
• Transgene: Tg(Col2a1-PTHLH)1Ecw Location: unknown
• Alliance: Tg(Col2a1-PTHLH)1Ecw page

Subgross analysis of skeletons of Tg(Col2a1-PTHLH)1Ecw/0 mice

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Col2a1-PTHLH)1Ecw expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PTHLH (5744)

  Pthlh

  (MGI:97800)

• Mutation details: A 568 bp fragment of human PTHLH cDNA under the control of the mouse Col2a1 promoter was used as the transgene. The Col2a1 promoter sequences were used to target expressioin of the transgene to proliferating and prehypertrophic chondrocytes. An artificial exon was engineered into the 5' end of the Col2a1 construct to prevent alternative splicing and the translation initiation codon was inactivated to ensure translation of the human PTHLH gene. 2.2 kb of human growth hormone untranslated sequences were added downstream of PTHLH to provide termination and polyadenylation signals as well as increase expression efficiency. Transgenic animals express human PTHLH at approximately 4x the amount of endogenous mouse protein. (J:76983)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:76983 Weir EC, et al., Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10240-5
• All: 5 reference(s)