Lat^tm1.1Mal
Targeted Allele Detail

Summary

• Symbol: Lat^tm1.1Mal
• Name: linker for activation of T cells; targeted mutation 1.1, Bernard Malissen
• MGI ID: MGI:2182312
• Synonyms: LatY136F, LAT^Y136F, Lat^Y6F
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^tm1.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77098
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Tyrosine codon 136 in exon 7 was changed to a phenylalanine (p.Y136F) through an A-to-T mutation. In addition to the intended mutation, a new Bgl II restriction enzyme cleavage site was introduced in the intron 3' of exon 7 to accommodate a loxP site flanked neomycin resistance gene. Founder mice were bred to deleter mice to eliminate the floxed neo cassette leaving a single loxP site in intron 7. Western blot analysis of CD4-positive T cells verified the presence of protein product in mutant animals. (J:77098)

Disease models

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 10 strains or lines available

References

• Original: J:77098 Aguado E, et al., Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science. 2002 Jun 14;296(5575):2036-40
• All: 21 reference(s)