Cyp7b1^tm1Lth
Targeted Allele Detail

Summary

• Symbol: Cyp7b1^tm1Lth
• Name: cytochrome P450, family 7, subfamily b, polypeptide 1; targeted mutation 1, Richard Lathe
• MGI ID: MGI:2182328
• Synonyms: Cyp7b1^-
• Gene: Cyp7b1 Location: Chr3:18126114-18297502 bp, - strand Genetic Position: Chr3, 4.98 cM
• Alliance: Cyp7b1^tm1Lth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77685
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Exon 2 was disrupted by the recombination of a construct containing IRES-lacZ and neo genes. Normal transcript was undetected by Northern blot analysis of homozygous mutant mice. Individual assays of various tissue homogenates showed a lack of DHEA hydroxylation, indicating the functional ablation of the encoded protein in homozygous mutant mice. The results obtained from beta-galactose staining differed from those of in situ hybridization. (J:77685)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp7b1 Mutation: 33 strains or lines available

References

• Original: J:77685 Rose K, et al., Neurosteroid hydroxylase CYP7B: vivid reporter activity in dentate gyrus of gene-targeted mice and abolition of a widespread pathway of steroid and oxysterol hydroxylation. J Biol Chem. 2001 Jun 29;276(26):23937-44
• All: 9 reference(s)