Cyp24a1^tm1Star
Targeted Allele Detail

Summary

• Symbol: Cyp24a1^tm1Star
• Name: cytochrome P450, family 24, subfamily a, polypeptide 1; targeted mutation 1, Rene St-Arnaud
• MGI ID: MGI:2182338
• Synonyms: 24-OHase
• Gene: Cyp24a1 Location: Chr2:170324877-170339065 bp, - strand Genetic Position: Chr2, 91.91 cM
• Alliance: Cyp24a1^tm1Star page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56453
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 9 and 10, the latter encoding the heme-binding domain, were replaced with a PGK-neo selection cassette. Transcript was reported to be undetectable in homozygous mutant mice. Neither an immunoassay nor HPLC fractionation followed by a competitive binding assay could detect the enzymatic product, 24,25-dihydroxyvitamin D, of the encoded protein in homozygous mutant mice. (J:63457)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp24a1 Mutation: 28 strains or lines available

References

• Original: J:56453 St-Arnaud R, Targeted inactivation of vitamin D hydroxylases in mice. Bone. 1999 Jul;25(1):127-9
• All: 7 reference(s)