Cyp1a2^tm1Dwn
Targeted Allele Detail

Summary

• Symbol: Cyp1a2^tm1Dwn
• Name: cytochrome P450, family 1, subfamily a, polypeptide 2; targeted mutation 1, Daniel W Nebert
• MGI ID: MGI:2182351
• Synonyms: Cyp1a2^-, HPRT KO BS-CL
• Gene: Cyp1a2 Location: Chr9:57584220-57590938 bp, - strand Genetic Position: Chr9, 31.3 cM
• Alliance: Cyp1a2^tm1Dwn page

Dioxin-induced hepatic injury in Cyp1a1^tm1.1Dwn/Cyp1a1^tm1.1Dwn and Cyp1a2^tm1Dwn/Cyp1a2^tm1Dwn mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31730
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of an hprt gene, with the opposite orientation of the endogenous gene, deleted a portion of exon 2 and all of exons 3 through 5. This deleted region encoded the conserved cysteine-containing fragment and the cytochrome P450 tridecapeptide. Northern blot analysis of total hepatic RNA obtained from beta-naphthoflavone-treated animals showed a lack of transcript in homozygous mutant mice. Failure of homozygous mutant mice to metabolize zoxazolamine, a muscle relaxant, indicated a lack of functional protein. (J:31730)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cyp1a2 Mutation: 37 strains or lines available

References

• Original: J:31730 Liang HC, et al., Cyp1a2(-/-) null mutant mice develop normally but show deficient drug metabolism. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1671-6
• All: 30 reference(s)