Pck1^tm1.1Lre
Targeted Allele Detail

Summary

• Symbol: Pck1^tm1.1Lre
• Name: phosphoenolpyruvate carboxykinase 1, cytosolic; targeted mutation 1.1, Lea Reshef
• MGI ID: MGI:2182394
• Synonyms: PPARE-
• Gene: Pck1 Location: Chr2:172994866-173001067 bp, + strand Genetic Position: Chr2, 95.79 cM, cytoband C1-qter
• Alliance: Pck1^tm1.1Lre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73977
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is a derivative of Pck1^tm1Lre in which Cre-mediated recombination removed the floxed PGK-neomycin resistance cassette that in the initial allele had replaced part of the adipose tissue-specific part of the promoter. Northern blot analysis of fasted homozygous mutant mice detected mRNA in liver and kidney, no mRNA in white adipose tissue, and a decreased level of mRNA in brown adipose tissue. RT-PCR studies using primers for exon 9 and 10 did not detect transcript in white adispose tissue from fasted homozygous mutant mice. (J:73977)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pck1 Mutation: 47 strains or lines available

References

• Original: J:73977 Olswang Y, et al., A mutation in the peroxisome proliferator-activated receptor gamma-binding site in the gene for the cytosolic form of phosphoenolpyruvate carboxykinase reduces adipose tissue size and fat content in mice. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):625-30
• All: 4 reference(s)