Dld^tm1Ptl
Targeted Allele Detail

Summary

• Symbol: Dld^tm1Ptl
• Name: dihydrolipoamide dehydrogenase; targeted mutation 1, Mulchand S Patel
• MGI ID: MGI:2182403
• Synonyms: Dld^-, Dld^tm1mjp
• Gene: Dld Location: Chr12:31381561-31401470 bp, - strand Genetic Position: Chr12, 13.43 cM
• Alliance: Dld^tm1Ptl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45074
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 10 was disrupted by the insertion of a neomycin selection cassette. Northern blot analysis of total hepatic and renal RNA showed that transcript levels are reduced by approximately 50% in heterozygous mice. RT-PCR analysis confirmed that the neo transgene was not excised via an altered splicing pattern. (J:45074)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dld Mutation: 20 strains or lines available

References

• Original: J:45074 Johnson MT, et al., Targeted disruption of the murine dihydrolipoamide dehydrogenase gene (Dld) results in perigastrulation lethality. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14512-7
• All: 6 reference(s)