Ncam1^tm1Mag
Targeted Allele Detail

Summary

• Symbol: Ncam1^tm1Mag
• Name: neural cell adhesion molecule 1; targeted mutation 1, Terry Magnuson
• MGI ID: MGI:2182406
• Synonyms: Ncam-180-, Ncam^tm1Cwr
• Gene: Ncam1 Location: Chr9:49413436-49710225 bp, - strand Genetic Position: Chr9, 26.83 cM
• Alliance: Ncam1^tm1Mag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37891
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A FRT-flanked neomycin resistance cassette driven by beta-actin replaced exon 18, which is specific to the 180 kDa isoform of the protein. Immunoblot analysis of brain tissues from homozygous mutant mice did not detect the 180 kDa isoform in neonatal and adult tissues, and detected the 140kDa isoform in neonatal tissues, but not in adult tissues. (J:37891)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncam1 Mutation: 65 strains or lines available

References

• Original: J:37891 Tomasiewicz H, et al., Genetic deletion of a neural cell adhesion molecule variant (N-CAM-180) produces distinct defects in the central nervous system. Neuron. 1993 Dec;11(6):1163-74
• All: 12 reference(s)