Dnmt3a^tm1Enl
Targeted Allele Detail

Summary

• Symbol: Dnmt3a^tm1Enl
• Name: DNA methyltransferase 3A; targeted mutation 1, En Li
• MGI ID: MGI:2182408
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^tm1Enl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58298
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.3kb region encoding the PC and ENV motifs was replaced with a cassette containing an IRES-lacZ gene and a neo gene. Northern blot analysis of total RNA showed an absence of transcript in homozygous mutant mice. X-gal staining of heterozygous embryos revealed expression of beta-galactosidase. (J:58298)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 139 strains or lines available

References

• Original: J:58298 Okano M, et al., DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999 Oct 29;99(3):247-57
• All: 7 reference(s)