Dnmt3b^tm2Enl
Targeted Allele Detail

Summary

• Symbol: Dnmt3b^tm2Enl
• Name: DNA methyltransferase 3B; targeted mutation 2, En Li
• MGI ID: MGI:2182410
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^tm2Enl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58298
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeting took place in J1 ES cells heterozygous for Dnmt3b^tm1Enl. A 2.4kb region encoding the PC and ENV motifs was replaced with a hygromycin selection cassette via homologous recombination. (J:58298)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 52 strains or lines available

References

• Original: J:58298 Okano M, et al., DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999 Oct 29;99(3):247-57
• All: 5 reference(s)