Dio2^tm1Vag
Targeted Allele Detail

Summary

• Symbol: Dio2^tm1Vag
• Name: deiodinase, iodothyronine, type II; targeted mutation 1, Valerie Anne Galton
• MGI ID: MGI:2182450
• Synonyms: D2KO, Dio2^-
• Gene: Dio2 Location: Chr12:90691326-90705812 bp, - strand Genetic Position: Chr12, 44.05 cM
• Alliance: Dio2^tm1Vag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72958
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a 2.6kb region including a 5' portion of exon 2. 72% of the coding region was deleted, including a TGA codon that encodes the selenocysteine located at the active site. Northern blot analysis of total RNA obtained from brown adipose tissue showed an absence of transcript in homozygous mutant mice. Functional ablation of the endogenous protein in homozygous mutant mice was confirmed by assaying deiodination in brown adipose tissue. (J:72958)

Expression

In Mice Carrying this Mutation:	114 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dio2 Mutation: 19 strains or lines available

References

• Original: J:72958 Schneider MJ, et al., Targeted Disruption of the Type 2 Selenodeiodinase Gene (DIO2) Results in a Phenotype of Pituitary Resistance to T(4). Mol Endocrinol. 2001 Dec;15(12):2137-48
• All: 40 reference(s)