Dlg4^tm1Grnt
Targeted Allele Detail

Summary

• Symbol: Dlg4^tm1Grnt
• Name: discs large MAGUK scaffold protein 4; targeted mutation 1, Seth G N Grant
• MGI ID: MGI:2182460
• Synonyms: PSD-95^-, PSD-95^PDZ12
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^tm1Grnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77693
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A stop codon was introduced into sequence encoding the PDZ3 domain. In addition, a cassette containing IRES-lacZ and neo was inserted downstream to replace sequence putatively essential for subcellular localization. An RNAse protection verified transcription of the targeted allele. While low levels of the mutant protein containing the PDZ1 and PDZ2 domains were detected by Western blot analysis of homozygous mutant forebrain extracts, the mutant protein was undetected in the synaptosome, synaptic plasma membrane, and posynaptic density fractions. Comparisons with previous results of in situ hybridization studies showed the expression pattern of beta-galactosidase to resemble that of the endogenous gene. (J:77693)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 46 strains or lines available

References

• Original: J:77693 Migaud M, et al., Enhanced long-term potentiation and impaired learning in mice with mutant postsynaptic density-95 protein. Nature. 1998 Dec 3;396(6710):433-9
• All: 24 reference(s)