Piwil1^tm1Hfl
Targeted Allele Detail

Summary

• Symbol: Piwil1^tm1Hfl
• Name: piwi-like RNA-mediated gene silencing 1; targeted mutation 1, Haifan Lin
• MGI ID: MGI:2182488
• Synonyms: miwi^null
• Gene: Piwil1 Location: Chr5:128813135-128832538 bp, + strand Genetic Position: Chr5, 67.86 cM
• Alliance: Piwil1^tm1Hfl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77015
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 15.5 kb of the gene spanning exons 3-22 were replaced with a loxP-flanked PGK-neo cassette preceded by a GFP reporter gene. The targeting event results in a GFP fusion product after the first 9 amino acids of the gene. Western blot and immunofluorescent microscopy of testis from homozygous mutant animals verified the absence of Piwil1 expression. (J:77015)

Expression

In Mice Carrying this Mutation:	29 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Piwil1 Mutation: 28 strains or lines available

References

• Original: J:77015 Deng W, et al., miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell. 2002 Jun;2(6):819-30
• All: 22 reference(s)