Tbpl1^tm1Saco
Targeted Allele Detail

Summary

• Symbol: Tbpl1^tm1Saco
• Name: TATA box binding protein-like 1; targeted mutation 1, Paolo Sassone-Corsi
• MGI ID: MGI:2182539
• Synonyms: TLF-
• Gene: Tbpl1 Location: Chr10:22579778-22607837 bp, - strand Genetic Position: Chr10, 10.25 cM, cytoband A3
• Alliance: Tbpl1^tm1Saco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68789
• Parent Cell Line: H1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by insertion of a loxP-flanked PGK-hygro cassette into exon 4 via homologous recombination. The gene targeting event results in truncation of the protein at amino acid 88 and elimination of the DNA binding surface. Gene expression is strongly decreased in brain and testes of homozygous mutant animals as determined by Northern blot. Protein product was not detected in testes of homozygous mutants as determined by Western blot. (J:68789)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbpl1 Mutation: 10 strains or lines available

References

• Original: J:68789 Martianov I, et al., Late arrest of spermiogenesis and germ cell apoptosis in mice lacking the TBP-like TLF/TRF2 gene. Mol Cell. 2001 Mar;7(3):509-15
• All: 3 reference(s)