Itgb8^tm1Lfr
Targeted Allele Detail

Summary

• Symbol: Itgb8^tm1Lfr
• Name: integrin beta 8; targeted mutation 1, Louis F Reichardt
• MGI ID: MGI:2182548
• Synonyms: beta8^-
• Gene: Itgb8 Location: Chr12:119121757-119202537 bp, - strand Genetic Position: Chr12, 63.48 cM
• Alliance: Itgb8^tm1Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77682
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo-cassette replaced sequences encoding 45 amino acids at the C-terminus, corresponding to exon 4, which includes a von Willebreand factor-A-like domain, and part of the intron sequences. This targeting event resulted in a frameshift in addition to a deletion, abolishing functional Itgb8 protein. Northern blot and RT-PCR analysis verified the lack of functional transcript in homozygous mice. (J:77682)

Expression

In Mice Carrying this Mutation:	39 assay results
In Structures Affected by this Mutation:	17 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itgb8 Mutation: 44 strains or lines available

Notes

ES cell line = JMA7.

References

• Original: J:77682 Zhu J, et al., beta8 integrins are required for vascular morphogenesis in mouse embryos. Development. 2002 Jun;129(12):2891-903
• All: 20 reference(s)