Ddx4^tm1Tnc
Targeted Allele Detail

Summary

• Symbol: Ddx4^tm1Tnc
• Name: DEAD box helicase 4; targeted mutation 1, Toshiaki Noce
• MGI ID: MGI:2182568
• Synonyms: Mvh^-, Mvh¹⁰⁹⁸
• Gene: Ddx4 Location: Chr13:112734867-112789009 bp, - strand Genetic Position: Chr13, 63.87 cM
• Alliance: Ddx4^tm1Tnc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61665
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4.5 kb region of the gene spanning exons 9 and 10 encoding the ATPase domain was replaced with a IRES-lacZ-PGK-neo cassette via homologous recombination. No gene transcripts were detected by RT-PCR analysis using primers amplifying exon 8 or 11, and Western blot analysis showed complete absence of protein product from testes of homozygous mutants. (J:61665)

Expression

In Mice Carrying this Mutation:	26 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx4 Mutation: 59 strains or lines available

References

• Original: J:61665 Tanaka SS, et al., The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000 Apr 1;14(7):841-53
• All: 9 reference(s)