Dsp^tm2Efu
Targeted Allele Detail

Summary

• Symbol: Dsp^tm2Efu
• Name: desmoplakin; targeted mutation 2, Elaine Fuchs
• MGI ID: MGI:2182575
• Synonyms: DP(-)
• Gene: Dsp Location: Chr13:38335357-38382553 bp, + strand Genetic Position: Chr13, 18.0 cM
• Alliance: Dsp^tm2Efu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51903
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced sequence encoding amino acids 281 through 474. The absence of transcript in homozygous mice was verified by Northern blot analysis. Immunohistochemical analysis using an antibody targeted to the amino terminal shows that neither normal or truncated protein was produced from the targeted allele. (J:51903)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dsp Mutation: 136 strains or lines available

References

• Original: J:51903 Gallicano GI, et al., Desmoplakin is required early in development for assembly of desmosomes and cytoskeletal linkage. J Cell Biol. 1998 Dec 28;143(7):2009-22
• All: 2 reference(s)