Fes^tm2Mcs
Targeted Allele Detail

Summary

• Symbol: Fes^tm2Mcs
• Name: feline sarcoma oncogene; targeted mutation 2, M Celeste Simon
• MGI ID: MGI:2182589
• Synonyms: c-fes^deltac
• Gene: Fes Location: Chr7:80027504-80037694 bp, - strand Genetic Position: Chr7, 45.65 cM
• Alliance: Fes^tm2Mcs page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:76509
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.5 kb region of the gene spanning exons 12-14 were replaced with a PGK-neo cassette via homologous recombination. The gene targeting event results in deletion of the C-terminal SH2 domain and the ATP-binding site of the tyrosine kinase domain (amino acids 497-607). Homozygous mutant embryonic stem cells were identified via Southern blot analysis. (J:76509)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fes Mutation: 41 strains or lines available

References

• Original: J:76509 Hackenmiller R, et al., Truncation of c-fes via gene targeting results in embryonic lethality and hyperproliferation of hematopoietic cells. Dev Biol. 2002 May 15;245(2):255-69
• All: 1 reference(s)