Hsf2^tm1Mmr
Targeted Allele Detail

Summary

• Symbol: Hsf2^tm1Mmr
• Name: heat shock factor 2; targeted mutation 1, Michel Morange
• MGI ID: MGI:2182620
• Synonyms: Hsf2^-
• Gene: Hsf2 Location: Chr10:57362481-57389231 bp, + strand Genetic Position: Chr10, 29.19 cM, cytoband B3-B4
• Alliance: Hsf2^tm1Mmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76942
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The gene is disrupted by insertion of a lacZ-neo cassette into exon 5 via homologous recombination. The gene targeting event results in the disruption of the oligomerization domain at lysine 167 and disruption of one of two nuclear localization signals while HSE-binding activity is retained. The mutant protein is expected to be cytoplasmic and lack trimerization abilities. RT-PCR and Western blot analyses confirmed the absence of gene expression in testes of homozygous mutant animals and homozygous mutant embryos. (J:76942)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hsf2 Mutation: 38 strains or lines available

References

• Original: J:76942 Kallio M, et al., Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice. EMBO J. 2002 Jun 3;21(11):2591-601
• All: 6 reference(s)