Efnb2^tm1Kln
Targeted Allele Detail

Summary

• Symbol: Efnb2^tm1Kln
• Name: ephrin B2; targeted mutation 1, Rudiger Klein
• MGI ID: MGI:2182626
• Synonyms: ephrinB2^-, ephrinB2 knockout, ephrinB2^KO, ephrinB2 null
• Gene: Efnb2 Location: Chr8:8667434-8711242 bp, - strand Genetic Position: Chr8, 3.42 cM
• Alliance: Efnb2^tm1Kln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53085
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The locus was disrupted by the insertion of a construct, containing a floxed neo gene and a lacZ gene fused to the transmembrane domain of the mouse Ntrk2 gene. The insertion was made just downstream of the sequences encoding the signal peptide. RT-PCR analysis confirmed the absence of transcript in homozygous mutant mice. Staining of heterozygous embyros showed expression of beta-galactosidase. (J:53085)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb2 Mutation: 29 strains or lines available

References

• Original: J:53085 Adams RH, et al., Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 1999 Feb 1;13(3):295-306
• All: 3 reference(s)