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Efnb2tm3.1Kln
Targeted Allele Detail
Summary
Symbol: Efnb2tm3.1Kln
Name: ephrin B2; targeted mutation 3.1, Rudiger Klein
MGI ID: MGI:2182632
Synonyms: ephrinB2deltaC
Gene: Efnb2  Location: Chr8:8667434-8711242 bp, - strand  Genetic Position: Chr8, 3.42 cM
Alliance: Efnb2tm3.1Kln page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:67296
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsMice heterozygous for Efnb2tm3Kln were bred with mice expressing a cre recombinase transgene, resulting in the excision of the floxed neo gene. The inserted cDNA encoding the truncated protein lacking 66 residues of carboxy terminal region and containing a hemagglutinin (HA) epitope tag, was left intact. The expression pattern of the targeted allele was determined to resemble that of the wild-type allele via in situ hybridization. Analysis using modified receptors showed localization to the cell surface. Production of protein with a reduced molecular weight was verified by immunoblotting with an anti-HA antibody. (J:67296)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Efnb2 Mutation:  29 strains or lines available
References
Original:  J:67296 Adams RH, et al., The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration. Cell. 2001 Jan 12;104(1):57-69
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory