Per1^tm1Saco
Targeted Allele Detail

Summary

• Symbol: Per1^tm1Saco
• Name: period circadian clock 1; targeted mutation 1, Paolo Sassone-Corsi
• MGI ID: MGI:2182634
• Synonyms: mPer1^-, Per1-
• Gene: Per1 Location: Chr11:68986043-69000786 bp, + strand Genetic Position: Chr11, 42.37 cM, cytoband B
• Alliance: Per1^tm1Saco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70850
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A region encompassing sequence from exons 4 through 10 was replaced with a neomycin selection cassette. The deleted region encoded the entire PAS domain and a downstream portion of the PAC domain that is involved in dimerization and regulatory functions. RT-PCR analysis showed the presence of two truncated transcripts in homozygous mutant mice. Sequence analysis of the transcripts showed that the inserted neomycin transgene had been excised and in-frame stop codons had been introduced. The presence of two transcripts was the result of different splice patterns, one which included the remaining 5' portion of exon 4 and one that did not. Western blot analysis of protein extracts showed an absence of encoded protein in homozyous mutant mice. (J:70850)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Per1 Mutation: 63 strains or lines available

References

• Original: J:70850 Cermakian N, et al., Altered behavioral rhythms and clock gene expression in mice with a targeted mutation in the Period1 gene. EMBO J. 2001 Aug 1;20(15):3967-74
• All: 3 reference(s)