Drgx^tm1And
Targeted Allele Detail

Summary

• Symbol: Drgx^tm1And
• Name: dorsal root ganglia homeobox; targeted mutation 1, David J Anderson
• MGI ID: MGI:2182636
• Synonyms: Drg11^-, Prrxl1^-
• Gene: Drgx Location: Chr14:32321364-32371203 bp, + strand Genetic Position: Chr14, 19.4 cM
• Alliance: Drgx^tm1And page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70560
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 were replaced with a IRES-Tau-lacZ-neo cassette via homologous recombination. The gene targeting event results in deletion of most of the paired-like homeodomain and putative DNA-binding domain. RT-PCR analysis of homozygous mutant animals was unable to detect gene transcription using primers to the gene coding region or neomycin resistance gene. Amplification products were obtained using primers to the lacZ gene, but lack of beta-gal activity suggests these transcripts were spliced abnormally. (J:70560)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Drgx Mutation: 13 strains or lines available

References

• Original: J:70560 Chen Z, et al., The paired homeodomain protein drg11 is required for the projection of cutaneous sensory afferent fibers to the dorsal spinal cord. Neuron. 2001 Jul 19;31(1):59-73
• All: 8 reference(s)