S1pr3^tm1Jch
Targeted Allele Detail

Summary

• Symbol: S1pr3^tm1Jch
• Name: sphingosine-1-phosphate receptor 3; targeted mutation 1, Jerold Chun
• MGI ID: MGI:2182637
• Synonyms: lpB3^-, s1p3^-
• Gene: S1pr3 Location: Chr13:51562675-51576833 bp, + strand Genetic Position: Chr13, 26.12 cM, cytoband B1
• Alliance: S1pr3^tm1Jch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71372
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Sequence encompassing the entire open reading frame, which is contained in the 5' region of exon 2, was flanked by an upstream loxP site in intron 1 and a downstream floxed neo cassette. The entire open reading frame and the neo transgene were excised via cre recombinase mediated deletion. Northern blot analysis and in situ hybridization showed an absence of transcript in homozygous mutant mice. A complete absence of encoded protein was determined via Western blot analysis using antibodies for the amino and carboxy termini. (J:71372)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any S1pr3 Mutation: 24 strains or lines available

References

• Original: J:71372 Ishii I, et al., Selective loss of sphingosine 1-phosphate signaling with no obvious phenotypic abnormality in mice lacking its G protein-coupled receptor, LP(B3)/EDG-3. J Biol Chem. 2001 Sep 7;276(36):33697-704
• All: 31 reference(s)