Itgb1^tm1Ehi
Targeted Allele Detail

Summary

• Symbol: Itgb1^tm1Ehi
• Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Emilio Hirsch
• MGI ID: MGI:2182640
• Synonyms: Beta(1de6)
• Gene: Itgb1 Location: Chr8:129412135-129459681 bp, + strand Genetic Position: Chr8, 76.09 cM, cytoband E2
• Alliance: Itgb1^tm1Ehi page

Developmental defects in Itgb1^tm1Ehi/Itgb1^tm1Ehi embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76216
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A duplication of exon 6 (an exon common to all known splice variants) was inserted into intron 6 along with stop codons, a polyadenylation signal, and a neo gene. A frameshift arises from the splicing of exon 6 to its duplicate, resulting in the substitution of the isoform-specific carboxy terminal sequences with a new carboxy terminal sequence encoding 11 residues. RT-PCR, immunoprecipitation, and immunolabeling analyses showed that the mutant isoform was the only isoform produced and that the altered carboxy terminal region did not affect localization of the protein in homozygous mutant mice. (J:76216)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb1 Mutation: 60 strains or lines available

References

• Original: J:76216 Hirsch E, et al., Defective Rac-mediated proliferation and survival after targeted mutation of the beta1 integrin cytodomain. J Cell Biol. 2002 Apr 29;157(3):481-92
• All: 1 reference(s)