Gadd45a^tm1Ajf
Targeted Allele Detail

Summary

• Symbol: Gadd45a^tm1Ajf
• Name: growth arrest and DNA-damage-inducible 45 alpha; targeted mutation 1, Albert J Fornace
• MGI ID: MGI:2182720
• Synonyms: Gadd45a-
• Gene: Gadd45a Location: Chr6:67012080-67014391 bp, - strand Genetic Position: Chr6, 30.72 cM
• Alliance: Gadd45a^tm1Ajf page

Exencephaly in Brca1^tm2.1Cxd/Brca1^tm2.1Cxd Gadd45a^tm1Ajf/Gadd45a^tm1Ajf embryos

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57882
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2.5 kb of the promoter and exons 1-3 were replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutants was verified by RNase protection assay using an exon 3 probe and mouse embryonic fibroblasts derived from E13.5 homozygous mutant embryos. (J:57882)

Disease models

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gadd45a^tm1Ajf

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gadd45a Mutation: 15 strains or lines available

Notes

ES cell line = ESVJ-1183 (129X1/SvJ) or HM-1 (129P2/OlaHsd).

References

• Original: J:57882 Hollander MC, et al., Genomic instability in Gadd45a-deficient mice. Nat Genet. 1999 Oct;23(2):176-84
• All: 38 reference(s)